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      • [PDF] Liver Stem Cells: Methods and Protocols Free Download by | Publisher : Humana Press | Category : Science | Tags : Biology, Models, Technology, Ips, Physiology, Matrix | ISBN-10 : 1617794678 | ISBN-13 : 9781617794674
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      • Liver Stem Cells: Methods and Protocols by , Publisher : Humana Press
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      • Liver Stem Cells: Methods and Protocols (Methods in Molecular Biology, Vol. 826)

        Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells: Methods and Protocols, expert researchers focus on  several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and  application of liver stem cells for cell therapy.  These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

        Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries.

        Table of Contents

        Part I: Several Hepatic Progenitor Cells
        Chapter 1: Purification and Culture of Fetal Mouse Hepatoblasts that Are Precursors of Mature Hepatocytes and Biliary Epithelial Cells
        Chapter 2: Clinical Uses of Liver Stem Cells
        Chapter 3: Identification and Isolation of Adult Liver Stem/Progenitor Cells
        Chapter 4: Isolation and Purification Method of Mouse Fetal Hepatoblasts
        Chapter 5: Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver

        Part II: Hepatic Differentiation from Stem Cells
        Chapter 6: Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
        Chapter 7: Development of Immortalized Hepatocyte-Like Cells from hMSCs
        Chapter 8: Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and Their Application to Liver Damages
        Chapter 9: Generation and Hepatic Differentiation of Human iPS Cells
        Chapter 10: Efficient Hepatic Differentiation from Human iPS Cells by Gene Transfer
        Chapter 11: “Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor
        Chapter 12: SAMe and HuR in Liver Physiology

        Part III: BD Formation from Stem Cells
        Chapter 13: Transdifferentiation of Mature Hepatocytes into Bile Duct/ductule Cells Within a Collagen Gel Matrix
        Part IV: Liver Stem Cells and Hepatocarcinogenesis
        Chapter 14: Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma

        Part V: Application of Liver Stem Cells for Cell Therapy
        Chapter 15: Intravenous Human Mesenchymal Stem Cells Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage
        Chapter 16: Engineering of Implantable Liver Tissues
        Chapter 17: Mesenchymal Stem Cell Therapy on Murine Model of Nonalcoholic Steatohepatitis

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    • Book Details
      • Book Name : Liver Stem Cells: Methods and Protocols

        Edition : 2012

        Author :

        Publisher : Humana Press

        Category : Science

        ISBN-10 : 1617794678

        ISBN-13 : 9781617794674

        ASIN : 1617794678

        Pages : 225

        Language : English

        Publish Date : December 17, 2011
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